Reporter

Part:BBa_J70563:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-17)


J04430 with C-terminal 6 his tag and no terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

designed


Source

digest J70562 with BsaXI and insert this to add a C-terminal 6 his tag

forward (J70562-f): aaGGATCCCACCACCACCACCACCACTAATAAtactagtagcggc

reverse (J70562-r): gctactagtaTTATTAGTGGTGGTGGTGGTGGTGGGATCCtttgt

References